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Location： Home > Polyclonal Antibodies > Anti-LIMK1(Ab-508) antibody

Anti-LIMK1(Ab-508) antibody#21151

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Product Detail

Product NameAnti-LIMK1(Ab-508) antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeImmunogen-specific rabbit IgG

PurificationAntigen affinity purification

ApplicationsWB IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescSynthetic peptide of human LIMK1

ConjugateUnconjugated

Other NamesLIMK; LIMK-1

Accession NoSwiss-Prot#:P53667

Uniprot P53667

Calculated MW73 kDa

Concentration0.8mg/ml

FormulationpH7.4 PBS, 0.05% NaN3, 40% Glycerol

Storage'-20°C

Application Details

WB dilution: 500-2000

IHC dilution: 30-150

Gel: 8%SDS-PAGELysate: 40 μgLane: HUVEC cell lysatePrimary antibody: LIMK1(Ab-508) Antibody at dilution 1/350Secondary antibody: Goat anti rabbit IgG at 1/8000 dilutionExposure time: 30 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using LIMK1(Ab-508) Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

 

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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