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CDC2(Ab-161) Antibody#21152

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Product Detail

Product NameCDC2(Ab-161) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total CDC2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.159~163 (T-Y-T-H-E) derived from Human CDC2.

Target NameCDC2

ConjugateUnconjugated

Other NamesCDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;

Accession NoSwiss-Prot: P06493
NCBI Protein: NP_001163877.1

Uniprot P06493

Gene ID 983;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 34kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from COLO cells using CDC2(Ab-161) Antibody #21152 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed Hela cells using CDC2(Ab-161) Antibody #21152.
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6
Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53
Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56
Liu Y, et al.(2004) J Biol Chem; 279(6): 4507-14

If you have published an article using product 21152, please notify us so that we can cite your literature.

et al,Sinularin induces oxidative stress鈥恗ediated G2/M arrest and apoptosis in oral cancer cells.In Environ Toxicol on 2017 Sep by Yung-Ting Chang , Chang-Yi Wu,et al..PMID:28548367 , (2017),
PMID: 28548367

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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