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Akt2(Ab-474) Antibody#21155

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Product Detail

Product NameAkt2(Ab-474) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Akt2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 472~476 (Q-F-S-Y-S) derived from Human Akt2.

Target NameAkt2

ConjugateUnconjugated

Other NamesPKB beta; Akt-2; Protein kinase B; RAC-PK-beta;

Accession NoSwiss-Prot: P31751
NCBI Protein: NP _001617.1

Uniprot P31751

Gene ID 208;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 and PC12 cells using Akt2(Ab-474) Antibody #21155.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2(Ab-474) Antibody #21155(left) or the same antibody preincubated with blocking peptide(right).
General protein kinase capable of phosphorylating several known proteins.

If you have published an article using product 21155, please notify us so that we can cite your literature.

et al,Girdin/GIV is upregulated by cyclic tension, propagates mechanical signal transduction, and is required for the cellular proliferation and migration of MG-63 cells.In Biochem Biophys Res Commun.On 2015 Aug 21 by Hu JT, Li Y et al..PMID:26163263 , (2015),
PMID: 26163263
et al,Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987 , (2012),
PMID: 23191987

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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