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Myosin Light Chain 2 (Ab-19) Antibody#21157

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Product Detail

Product NameMyosin Light Chain 2 (Ab-19) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Myosin Light Chain 2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.17~21 (A-T-S-N-V) derived from Human Myosin Light Chain 2.

Target NameMLC2

ConjugateUnconjugated

Other NamesLC20, MLC2, MRLC1, MYRL2, MLC-2C

Accession NoSwiss-Prot: P24844
NCBI Protein: NP_006088.2

Uniprot P24844

Gene ID 10398;

Sdspage MW18kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Immunofluorescence staining of methanol-fixed Hela cells using Myosin Light Chain 2(Ab-19) Antibody #21157.
Western blot analysis of extracts from293 and 3T3 cells using Myosin Light Chain 2 (Ab-19) Antibody #21157.

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion

Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619.
Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627
Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256

If you have published an article using product 21157, please notify us so that we can cite your literature.

et al,Tetramethylpyrazine Suppresses the Enhanced Ca2+ Sensitivity through Inhibiting the Expression of RhoA-ROCK in Artery of Simulated Weightlessness Rats. , (2022),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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