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nNOS(Ab-852) Antibody#21159

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Product Detail

Product NamenNOS(Ab-852) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total nNOS protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.850~854 (F-N-S-V-S) derived from Human nNOS.

Target NamenNOS

ConjugateUnconjugated

Other NamesBNOS; Constitutive NOS; N-NOS; NC-NOS; NOS1

Accession NoSwiss-Prot: P29475
NCBI Protein: NP_000611.1

Uniprot P29475

Gene ID 4842;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 160kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Rat and Mouse brain tissue using nNOS(Ab-852) Antibody #21159.
Immunofluorescence staining of methanol-fixed Hela cells using nNOS(Ab-852) Antibody #21159.
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter.

Liu JL et al. (2008) World J Gastroenterol. 14(20):3242-8.
Guggilam A et al. (2008) Eur J Heart Fail. 10(7):625-34
Marquina M et al. (2008) Br J Dermatol.159(1):68-76.

If you have published an article using product 21159, please notify us so that we can cite your literature.

et al,Regulation of Tracheal Ciliary Beat Frequency by Nitric Oxide Synthase Substrate L-arginine.In ORL J Otorhinolaryngol Relat Spec on 2010 by Jian Jiao, Demin Han,et al..PMID:20110742 , (2010),
PMID: 20110742
et al,Neuronal nitric oxide synthase/reactive oxygen species pathway is involved in apoptosis and pyroptosis in epilepsyInNeural Regen ResOn2023 JunbyXiao-Xue Xu, Rui-Xue Shi et al..PMID:36453412 , (2023),
PMID: 36453412

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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