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PAK1(Ab-212) Antibody#21160

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Product Detail

Product NamePAK1(Ab-212) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total PAK1protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 210~214 (P-V-T-P-T) derived from Human PAK1.

Target NamePAK1

ConjugateUnconjugated

Other Namesp21-activated kinase 1

Accession NoSwiss-Prot: Q13153
NCBI Protein: NP_001122092.1

Uniprot Q13153

Gene ID 5058;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 and 3T3 cells using PAK1(Ab-212) Antibody #21160.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1(Ab-212) Antibody #21160(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PAK1(Ab-212) Antibody #21160.
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819
Thiel DA, et al. (2002) Curr Biol; 12:1227-1232
Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052.

If you have published an article using product 21160, please notify us so that we can cite your literature.

et al,Phosphorylation of histone H3 on Ser10 by auto-phosphorylated PAK1 is not essential for chromatin condensation and meiotic progression in porcine oocytes , (2013),
PMID: 23521812
et al, PAK1 Regulates Spindle Microtubule Organization During Oocyte Meiotic Maturation.In Front Biosci (Elite Ed) on 2010 Jun 1 by Sheng-Li Lin , Shu-Tao Qi,et al..PMID: 20515799 , (2010),
PMID: 20515799
et al,Uptake of Shiga鈥恡oxigenic Escherichia coli鈥匰ubAB by HeLa cells requires an actin锟?and lipid raft鈥恉ependent pathway.In Cell Microbiol on 2014 Oct by Sayaka Nagasawa 1 , Kohei Ogura et al..PMID:24844382 , (2014),
PMID: 24844382
et al,PAK1 Is Involved in the Spindle Assembly during the First Meiotic Division in Porcine OocytesInInt J Mol SciOn2023 Jan 6byLei Peng?1,?Yijing He et al..PMID: 36674642 , (2023),
PMID: 36674642

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;