Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Polyclonal Antibodies > AFX(Ab-197) Antibody

AFX(Ab-197) Antibody#21162

Availability: Yes
Total Amount:$
Product Detail

Product NameAFX(Ab-197) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total AFX protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 195~199 (A-A-S-M-D) derived from Human AFX.

Target NameAFX

ConjugateUnconjugated

Other NamesAFX; FOXO4; AFX1; Afxh;

Accession NoSwiss-Prot: P98177
NCBI Protein: NP_001164402.1

Uniprot P98177

Gene ID 4303;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 65kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 and THP1 cells using AFX(Ab-197) Antibody #21162.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AFX(Ab-197) Antibody #21162(left) or the same antibody preincubated with blocking peptide(right).
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Essers MA, et al. EMBO J 2004 Nov. 11.
Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46.
Kops GJ, et al. (1999) Nature; 398(6728): 630-4.

If you have published an article using product 21162, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;