Product Detail
Product Namecdc25A(Ab-76) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total cdc25A protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 73~77 (M-G-S-S-E) derived from Human cdc25A.
Target Namecdc25A
ConjugateUnconjugated
Other NamesCDC25M3; MPIP1;
Accession NoSwiss-Prot: P30304
NCBI Protein: NP_001780.2
Uniprot
P30304
Gene ID
993;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 70kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela and 3T6 cells using cdc25A(Ab-76) Antibody #21163.
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Jin J, et al. (2003) Genes 17(24): 3062-74.
Shimuta K, et al. (2002) EMBO J 21(14): 3694-703.
If you have published an article using product 21163, please notify us so that we can cite your literature.
Yes
