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Location： Home > Polyclonal Antibodies > cofilin(Ab-3) Antibody

cofilin(Ab-3) Antibody#21164

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Product Detail

Product Namecofilin(Ab-3) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total cofilin protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 1~5 (M-A-S-G-V) derived from Human cofilin.

Target Namecofilin

ConjugateUnconjugated

Other NamesCFL; CFL1; COF1; Cofilin;

Accession NoSwiss-Prot: P23528
NCBI Protein: NP_005498.1

Uniprot P23528

Gene ID 1072;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Western blot analysis of extracts from MDA cells using cofilin(Ab-3) Antibody #21164.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Ab-3) Antibody #21164(left) or the same antibody preincubated with blocking peptide(right).

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Kobayashi M, et al. (2006) EMBO J 25(4): 713-26.
Wang Y, et al. (2005)Biol Chem 280(13): 12683-9.
Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.
 

If you have published an article using product 21164, please notify us so that we can cite your literature.

et al,DGCR6L, a novel PAK4 interaction protein, regulates PAK4-mediated migration of human gastric cancer cell via LIMK1. In Int J Biochem Cell Biol on 2010 Jan by Xiaodong Li, Qiang Ke, et al..PMID: 19778628 , (2010),
PMID: 19778628
et al,Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis. In Development on 2011 Feb by Lijun Zhang, Jun Luo, et al..PMID: 21205790 , (2011),
PMID: 21205790
et al,A Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles for Actin Disassembly during Myocardial Growth and Maintenance in Mice.In Am J Pathol on 2014 Jul by Baiyin Yuan , Ping Wan et al..PMID: 24840128 , (2014),
PMID: 24840128
et al,Cofilin-mediated actin dynamics promotes actin bundle formation during Drosophila bristle development. In Mol Biol Cell on 2016 Aug 15 by Jing Wu, Heng Wang,et al..PMID:27385345 , (2016),
PMID: 27385345
et al,Fascin regulates nuclear actin during Drosophila oogenesis. In Mol Biol Cell on 2016 Oct 1 by Daniel J Kelpsch, Christopher M Groen,et al..PMID:27535426 , (2016),
PMID: 27535426
et al,Platelet-dependent signaling and Low Molecular Weight Protein Tyrosine Phosphatase expression promote aggressive phenotypic changes in gastrointestinal cancer cells. In Biochim Biophys Acta Mol Basis Dis on 2022 Jan 1 by Alessandra V S Faria, Bingting Yu, et al..PMID: 34610471 , (2022),
PMID: 34610471
et al,Physical Training vs. Perindopril Treatment on Arterial Stiffening of Spontaneously Hypertensive Rats: A Proteomic Analysis and Possible MechanismsInBiomedicinesOn2023 May 6byDanyelle Siqueira Miotto 1, Francine Duchatsch et al..PMID: 37239052 , (2023),
PMID: 37239052

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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