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Src(Ab-529) Antibody#21168

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Product Detail

Product NameSrc(Ab-529) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Src protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 527~531 (P-Q-Y-Q-P) derived from Human Src.

Target NameSrc

ConjugateUnconjugated

Other NamesC-SRC; SRC1;

Accession NoSwiss-Prot: P12931
NCBI Protein: NP_005408.1

Uniprot P12931

Gene ID 6714;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HT29, Hela and MDA cells using Src(Ab-529) Antibody #21168.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src(Ab-529) Antibody #21168(left) or the same antibody preincubated with blocking peptide(right).
Western Blot analysis of Hela /293 cells using Src Polyclonal Antibody

proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838
Pyper J.M.(1990) Mol. Cell. Biol. 10:2035-2040
Xu W., (1997).Nature 385:595-602
Benes C.H., (2005) Cell 121:271-280

If you have published an article using product 21168, please notify us so that we can cite your literature.

et al,PTBP3 contributes to the metastasis of gastric cancer by mediating CAV1 alternative splicing.In Cell Death Dis.On 2018 May 1 by PMID: 29752441 , (2018),
PMID: 29752441
et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861 , (2019),
PMID: 31594861
et al,FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST. In Mol Cell on 2009 Jul 10 by Yanhua Zheng, Yan Xia, et al..PMID:19595712 , (2009),
PMID: 19595712
et al,HER2-targeted hybrid peptide that blocks HER2 tyrosine kinase disintegrates cancer cell membrane and inhibits tumor growth in vivo. In Mol Cancer Ther on 2013 Apr by Megumi Kawamoto, Tomohisa Horibe, et al..PMID:23358664 , (2013),
PMID: 23358664
et al,PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis.In Nat Commun on 2016 Aug 3 by Ji Liang , Ruixiu Cao et al..PMID: 27485204 , (2016),
PMID: 27485204
et al,Sodium Bicarbonate Facilitates Hemostasis in the Presence of Cerebrospinal Fluid Through Amplification of Platelet Aggregation. In Neurosurgery on 2016 Feb by Yukinori Kozuma, Tetsuya Yamamoto,et al..PMID: 26457486 , (2016),
PMID: 26457486

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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