Product Detail
Product NameeNOS(Ab-1177) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total eNOS protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS.
Target NameeNOS
ConjugateUnconjugated
Other NamesConstitutive NOS; EC-NOS; ECNOS; NOS3; NOSIII
Accession NoSwiss-Prot: P29474
NCBI Protein: NP_000594.2
Uniprot
P29474
Gene ID
4846;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 140kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Fulton, D. et al. (1999) Nature 399, 597-601.
Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
If you have published an article using product 21170, please notify us so that we can cite your literature.
et al,Regulation of ciliary beat frequency by the nitric oxide signaling pathway in mouse nasal and tracheal epithelial cells.
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et al,Lipopolysaccharide Induces Subacute Cerebral Microhemorrhages with Involvement of Nitric Oxide Synthase in Rats. In J Stroke Cerebrovasc Dis.On 2018 Jul by Zeng J, Zh o H et al..PMID:29598904
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et al,Post-translational modification s of eNOS augment nitric oxide availability and facilitates hypoxia adaptation in Ladakhi women.In Nitric Oxide. 2018 On Aug 1 by Pooja, Ghosh D et al..PMID:29894791
, (2018),
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et al,Chronic exercise impairs nitric oxide pathway in rabbit carotid and femoral arteries.In J Physiol. On 2018 Sep by Marchio P, Guerra-Ojeda S et al..PMID:29968308
, (2018),
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et al,IcariIn reduces high glucose?Induced endothelial progenitor cell dysfunction via InhibitIng the p38/CREB pathway and activatIng the Akt/eNOS/NO pathway. In Exp Ther Med on 2019 Dec by Chen S, Wang Z, et al..PMID:31772646
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PMID:
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et al,Neuroprotective effects of troxerutin and cerebroprotein hydrolysate injection on the neurovascular unit in a rat model of Middle cerebral artery occlusion.In Int J Neurosci on 2020 Mar 31: by Zh o H, Wang R, et al..PMID: 32125198
, (2020),
PMID:
32125198
et al,Troxerutin cerebroprotein hydrolysate injection ameliorates neurovascular injury induced by traumatic brain injury via endothelial nitric oxide synthase pathway regulation .In Int J Neurosci. On 2018 Dec by Zh o H, Liu Y et al..PMID:29883225
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More
et al,Regulation of Ciliary Beat Frequency by the Nitric Oxide Signaling Pathway in Mouse Nasal and Tracheal Epithelial Cells.In Exp Cell Res on 2011 Oct 15 by Jian Jiao, Hong Wang, et al..PMID:21787770
, (2011),
PMID: 21787770
et al,Regulation of Tracheal Ciliary Beat Frequency by Nitric Oxide Synthase Substrate L-arginine.In ORL J Otorhinolaryngol Relat Spec
on 2010 by Jian Jiao, Demin Han,et al..PMID:20110742
, (2010),
PMID: 20110742
et al,Study of the Protective Mechanisms of Compound Danshen Tablet (Fufang Danshen Pian) Against Myocardial Ischemia/Reperfusion Injury via the Akt-eNOS Signaling Pathway in Rats.In J Ethnopharmacol on 2014 Oct 28 by Qin Ren-an, Lin Juan et al..PMID:25178948
, (2014),
PMID: 25178948
et al,Genipin derivatives protect RGC-5 from sodium nitroprusside-induced nitrosative stress. In Int J Mol Sci on 2016 Jan 19 by Rikang Wang, Jiaqiang Zhao,et al..PMID:26797604
, (2016),
PMID: 26797604
et al,Estrogen receptor (ESR1 and ESR2)-mediated activation of eNOS-NO-cGMP pathway facilitates high altitude acclimatization. In Nitric Oxide on 2020 Sep 1 by Pooja, Manish Sharma, et al..PMID:32544536
, (2020),
PMID: 32544536
et al,Association Between 17尾-Estradiol Receptors and Nitric Oxide Signaling Augments High-Altitude Adaptation of Ladakhi Highlanders. In High Alt Med Biol on 2021 Jun by
Pooja, Vandana Sharma, et al..PMID:33602001
, (2021),
PMID: 33602001
et al,Neuroprotective effects of troxerutin and cerebroprotein hydrolysate injection on the neurovascular unit in a rat model of Middle cerebral artery occlusion. In Int J Neurosci on
2021 Mar by H楂噂yi Zh锟? Ru Wang,et al..PMID:32125198
, (2021),
PMID: 32125198
et al,Deleterious effects of levamisole, a cocaine adulterant, in rabbit aorta. In Vascul Pharmacol on 2022 Jun by Sol Guerra-Ojeda, Patricia Marchio,et al..PMID:35358704
, (2022),
PMID: 35358704
et al,Levamisole Impairs Vascular Function by Blocking α-Adrenergic Receptors and Reducing NO Bioavailability in Rabbit Renal Artery. In Cardiovasc Toxicol on 2024 Aug by Sol Guerra-Ojeda, Patricia Marchio,et al..PMID:38877381
, (2024),
PMID: 38877381
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