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Zap-70(Ab-493) Antibody#21174

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Product Detail

Product NameZap-70(Ab-493) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Zap-70 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.491~495 (S-Y-Y-T-A) derived from Human ZAP70.

Target NameZap-70

ConjugateUnconjugated

Other Names70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70

Accession NoSwiss-Prot: P43403
NCBI Protein: NP _001070.2

Uniprot P43403

Gene ID 7535;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HL60 cells using Zap-70(Ab-493) Antibody #21174.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Zap-70(Ab-493) Antibody #21174(left) or the same antibody preincubated with blocking peptide(right).
ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions

Chan AC, et al. (1995) EMBO J; 14(11): 2499-2508
Kong G, et al. (1996) Mol Cell Biol; 16(9): 5026-5035
Salomon AR, et al. (2003)Proc Natl Acad Sci U S A; 100(2): 443-448

If you have published an article using product 21174, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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