Product Detail
Product NameMEK1(Ab-221) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total MEK1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.219~223 (A-N-S-F-V) derived from Human MEK1.
Target NameMEK1
ConjugateUnconjugated
Other NamesERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1
Accession NoSwiss-Prot: Q02750
NCBI Protein: NP_002746.1
Uniprot
Q02750
Gene ID
5604;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 45kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells using MEK1(Ab-221) Antibody #21175
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1(Ab-221) Antibody #21175(left) or the same antibody preincubated with blocking peptide(right).
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Zebisch A, et al. (2006) Cancer Res; 66(7): 3401-8.
Luciano BS, et al. (2006)J Biol Chem; 279(50): 52117-23.
Wang X, et al. (2003) Oncogene; 22(1): 109-16.
Gopalbhai K, et al. (2003) J Biol Chem; 278(10): 8118-25.
Ling MT, et al. (2002)Oncogene; 21(55): 8498-505.
If you have published an article using product 21175, please notify us so that we can cite your literature.
et al,MicroRNA-146b, a sensitive indicator of mesenchymal stem cell repair of acute renal injury. In Stem Cells Transl Med on 2016 Oct by Yuan Zhu, Jing Yu, et al..PMID: 27400799
, (2016),
PMID:
27400799
Yes
