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IkB-a(Ab-42) Antibody#21176

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Product Detail

Product NameIkB-a(Ab-42) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of total IkB-a protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 40~44 (E-E-Y-E-Q) derived from Human IkB-a.

Target NameIkB-a

ConjugateUnconjugated

Other NamesI-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA

Accession NoSwiss-Prot: P25963
NCBI Protein: NP_065390.1

Uniprot P25963

Gene ID 4792;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 39kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 and THP1 cells using IkB-a(Ab-42) Antibody #21176.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(Ab-42) Antibody #21176(left) or the same antibody preincubated with blocking peptide(right).
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

B

If you have published an article using product 21176, please notify us so that we can cite your literature.

et al,Perfluorocarbon Attenuates Lipopolysaccharide-Mediated Inflammatory Responses of Alveolar Epithelial Cells in Vitro.In Chin Med J (Engl) on 2011 Aug by Shu-Feng Xu, Ping Wang,et al..PMID:21933601 , (2011),
PMID: 21933601
et al,Clonorchis sinensis ferritin heavy chain triggers free radicals and mediates inflammation signaling in human hepatic stellate cells.In Parasitol Res on 2015 Feb by Qiang Mao, Zhizhi Xie et al..PMID: 25413629 , (2015),
PMID: 25413629

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;