Product Detail
Product NameDNA PKcs(Ab-2609) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total DNA PKcs protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.2607~2611 (V-E-T-Q-A) derived from Human DNA-PK.
Target NameDNA PKcs
ConjugateUnconjugated
Other NamesDNPK1; PRKD; PRKDC; XRCC7; P460
Accession NoSwiss-Prot: P78527
NCBI Protein: NP_001075109.1
Uniprot
P78527
Gene ID
5591;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 450kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 and HepG2 cells using DNA PKcs(Ab-2609) antibody #21179.
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties
Chan DW, et al. (2002) Genes Dev. Sep 15; 16(18): 2333-2338
Ding Q, et al. (2003) Mol Cell Biol. Aug; 23(16): 5836-5848
Douglas P, et al. (2002) Biochem J. Nov 15; 368(Pt 1): 243-251
If you have published an article using product 21179, please notify us so that we can cite your literature.
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