Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Polyclonal Antibodies > p70 S6 Kinase(Ab-389) Antibody

p70 S6 Kinase(Ab-389) Antibody#21182

Availability: Yes
Total Amount:$
Product Detail

Product Namep70 S6 Kinase(Ab-389) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total p70 S6 Kinase protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.387~391 (G-F-T-Y-V) derived from Human p70 S6 Kinase.

Target Namep70 S6 Kinase

ConjugateUnconjugated

Other NamesKS6B1; P70-S6K; RPS6KB1; S6K;

Accession NoSwiss-Prot: P23443
NCBI Protein: NP_003152.1

Uniprot P23443

Gene ID 6198;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 70 85 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells using p70 S6 Kinase(Ab-389) Antibody #21182(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunofluorescence staining of methanol-fixed Hela cells using p70 S6 Kinase(Ab-389) Antibody #21182.
Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851
Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.
Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c
Le, X.F, et al. (2003) Oncogene 22: 484

If you have published an article using product 21182, please notify us so that we can cite your literature.

et al,Long-term Stress with Hyperglucocorticoidemia-induced Hepatic Steatosis with VLDL Overproduction Is Dependent on both 5-HT2 Receptor and 5-HT Synthesis in Liver.In Int J Biol Sci on 2016 Jan 1 by Jihua Fu , Shaoxin Ma et al..PMID: 26884719 , (2016),
PMID: 26884719
et al,Effective treatment with combination of peripheral 5鈥恏ydroxytryptamine synthetic inhibitor and 5鈥恏ydroxytryptamine 2 receptor antagonist on glucocorticoid鈥恑nduced whole鈥恇ody insulin resistance with hyperglycemia.In J Diabetes Investig on 2016 Nov by Shaoxin Ma , Tao Li et al..PMID:27177506 , (2016),
PMID: 27177506
et al,Rapamycin enhances the anti-cancer effect of dasatinib by suppressing Src/PI3K/mTOR pathway in NSCLC cells. In PLoS One on 2015 Jun 10 by Bin Chen, Xin Xu,et al..PMID:26061184 , (2015),
PMID: 26061184

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;