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14-3-3z(Ab-58) Antibody#21188

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Product Detail

Product Name14-3-3z(Ab-58) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total 14-3-3 z protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta.

Target Name14-3-3z

ConjugateUnconjugated

Other Names1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1

Accession NoSwiss-Prot: P63104
NCBI Protein: NP _001129171.1

Uniprot P63104

Gene ID 7534;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 28kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HepG2 , Hela and 293 cells using 14-3-3z(Ab-58) Antibody #21188.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3z(Ab-58) Antibody #21188(left) or the same antibody preincubated with blocking peptide(right).
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Gu YM, et al. (2006) FEBS Lett ; 580(1): 305-310
Powell DW, et al. (2003) Mol Cell Biol; 23(15): 5376-5387
Mackintosh C. (2004) Biochem J; 381(Pt 2): 329-342.

If you have published an article using product 21188, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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