Product Detail
Product NameSMC1(Ab-957) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total SMC1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 955~959 (G-S-S-Q-G) derived from Human SMC1.
Target NameSMC1
ConjugateUnconjugated
Other NamesSM1A; SMC1A; SMC1L1; SMC1 alpha protein;
Accession NoSwiss-Prot: Q14683
NCBI Protein: NP_006297.2
Uniprot
Q14683
Gene ID
8243;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 145kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from JK cells using SMC1(Ab-957) Antibody #21190.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SMC1(Ab-957) Antibody #21190(left) or the same antibody preincubated with blocking peptide(right).
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Yang XM. et al. (2004) Mol Cell Biol. 24(12): 5290-5303.
Kim ST. et al. (2002) Genes Dev. 16(5): 560-570.
Revenkova E.et al. (2001) Mol Cell Biol. 21(20): 6984-6998
If you have published an article using product 21190, please notify us so that we can cite your literature.
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