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AMPKα1/AMPKα2 (Ab-183/172) Antibody#21191

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Product NameAMPKα1/AMPKα2 (Ab-183/172) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB,IHC,IF,ELISA

Species ReactivityHuman,Mouse,Rat,Monkey,Bovine,Fish

SpecificityThe antibody detects endogenous level of total AMPKa1/AMPKa2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 181~185/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2.

Target NameAMPKa1/AMPKa2

ConjugateUnconjugated

Other NamesAMPK, AMPKa1

Accession NoSwiss-Prot: Q13131
NCBI Protein: NP_006242.5 NP_006243.2

Uniprot Q13131

Gene ID 5562;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-183/172) Antibody #21191.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα1/AMPKα2 (Ab-183/172) Antibody #21191 (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed Hela cells using AMPKα1/AMPKα2(Ab-183/172) Antibody #21191.

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280(32): 29060-29066
Woods A, et al. (2003) Curr Biol. Nov 11; 13(22): 2004-2008
Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94(2): 631-641
Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97(8): 4023-4028.

If you have published an article using product 21191, please notify us so that we can cite your literature.

et al,Gemcitabine induces apoptosis and autophagy via the AMPK/mTOR signaling pathway in pancreatic cancer cells,In Biotechnol Appl Biochem. On 2018 Sep by Zhu J, Chen Y et al..PMID: 29575133 , (2018),
PMID: 29575133
et al,Metformin attenuates the D?galactose?induced aging process via the UPR through the AMPK/ERK1/2 signaling pathways.In Int J Mol Med on 2020 Mar by Cai H, Han B et al..PMID: 31922237 , (2020),
PMID: 31922237
et al,The Role of Sodium Hydrosulfide in Attenuating the Aging Process via PI3K/AKT and CaMKK尾/AMPK Pathways.In Redox Biol on 2017 Aug by Xubo Chen ,Xueyan Zhao ,et al..PMID: 28499253 , (2017),
PMID: 28499253
et al,Hydrogen Sulphide Treatment Increases Insulin Sensitivity and Improves Oxidant Metabolism Through the CaMKKbeta-AMPK Pathway in PA-Induced IR C2C12 Cells.In Sci Rep on 2017 Oct 16 by Xubo Chen, Xueyan Zhao,et al..PMID:29038536 , (2017),
PMID: 29038536
et al,FSTL1 as a Potential Mediator of Exercise-Induced Cardioprotection in Post-Myocardial Infarction Rats.In Sci Rep on 2016 Aug 26 by Yue Xi, Da-Wei Gong et al..PMID: 27561749 , (2016),
PMID: 27561749
et al,Stanniocalcin-1 inhibits renal ischemia/reperfusion injury via an AMP-activated protein kinase-dependent pathway. In J Am Soc Nephrol on 2015 Feb by Luping Huang, Tatiana Belousova, et al..PMID: 25012175 , (2015),
PMID: 25012175

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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