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Ephrin-B2(Ab-316) Antibody#21195

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Product Detail

Product NameEphrin-B2(Ab-316) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Ephrin-B2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.314~318 (P-V-Y-I-V) derived from Human Ephrin B (ephrin-B2).

Target NameEphrin-B2

ConjugateUnconjugated

Other NamesEFNB2; HTKL; LERK5

Accession NoSwiss-Prot: P52799
NCBI Protein: NP_004084.1

Uniprot P52799

Gene ID 1948;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 37kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from HUVEC cells using Ephrin-B2(Ab-316) Antibody #21195 and the same antibody preincubated with blocking peptide.

Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.

Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al.( 2006) Blood;107(6):2330-2338.
Noren NK, et al.( 2004) Proc Natl Acad Sci USA;101(15):5583-5588.

If you have published an article using product 21195, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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