Product Detail
                                
                                                                        Product NameBcr(Ab-177) Antibody
                                                                                 Host SpeciesRabbit
                                         ClonalityPolyclonal
                                                                                PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. 
                                        ApplicationsWB IF
                                        Species ReactivityHu Ms
                                        SpecificityThe antibody detects endogenous level of total Bcr protein.
                                        Immunogen TypePeptide-KLH
                                         Immunogen DescPeptide sequence around aa. 175~179 (P-F-Y-V-N) derived from Human Bcr.
                                         Target NameBcr
                                         ConjugateUnconjugated
                                                                                 Other NamesRenal carcinoma antigen NY-REN-26
                                        Accession NoSwiss-Prot: P11274
NCBI Protein: NP_004318.3 
                                        Uniprot
                                        P11274
                                        
                                        Gene ID
                                        613;
                                        
                                                                                                                        Concentration1.0mg/ml
                                         FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. 
                                        StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use. 
                                                                                                                                                         
                                Application Details
                                Predicted MW: 130 160 210kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
                             
                            
                                                                
                                    
                                    Western blot analysis of extracts from HL60 and K562 cells using Bcr(Ab-177) Antibody #21197.
                                 
                                                                
                                    
                                    Immunofluorescence staining of methanol-fixed Hela cells using Bcr(Ab-177) Antibody #21197.
                                 
                                                             
                            
                                GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Sattler M, et al. Mol Cell Biol. 1999 Nov; 19(11): 7473-7480.
Li S et al. EMBO J. 2001 Dec 3; 20(23): 6793-6804.
Million RP, et,al. Mol Cell Biol. 2004 Jun; 24(11): 4685-4695.
Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907 
                            
                            
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