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Paxillin(Ab-31) Antibody#21199

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Product Detail

Product NamePaxillin(Ab-31) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total Paxillin protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.29~33 (T-P-Y-S-Y) derived from Human Paxillin.

Target NamePaxillin

ConjugateUnconjugated

Other NamesPAXI; PXN;

Accession NoSwiss-Prot: P49023
NCBI Protein: NP_001074324.1

Uniprot P49023

Gene ID 5829;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 cells using Paxillin(Ab-31) Antibody #21199.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin(Ab-31) Antibody #21199(left) or the same antibody preincubated with blocking peptide(right).

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Davidson D, et al. (2001) EMBO J; 20(13): 3414-3426
Kook S, et al. (2000) Mol Biol Cell; 11(3): 929-939
Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96(3): 1123-1128
Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65(4): 595-626
Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96(15): 8449-8454

If you have published an article using product 21199, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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