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Ezrin(Ab-567) Antibody#21200

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Product Detail

Product NameEzrin(Ab-567) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Ezrin protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 565~569 (Y-K-T-L-R) derived from Human Ezrin.

Target NameEzrin

ConjugateUnconjugated

Other NamesCytovillin; EZRI; VIL2; Villin 2; p81

Accession NoSwiss-Prot: P15311
NCBI Protein: NP_001104547.1

Uniprot P15311

Gene ID 7430;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 81kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela,MDA and 3T3 cells using Ezrin(Ab-567) Antibody #21200.
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Zhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91.
Shiue H, et al.(2005) J Biol Chem: 280(2): 1688-95.
Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101(25): 9485-90.
Fievet BT, et al. (2004) J Cell Biol: 164(5): 653-9.

If you have published an article using product 21200, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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