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Rac1(Ab-71) Antibody#21201

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Product Detail

Product NameRac1(Ab-71) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAffinity purification

ApplicationsWB IF IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Rac1 protein.

Immunogen TypePeptide-KLH

Immunogen DescRecombinant fusion protein containing a sequence corresponding of human Rac(NP_008839.2).

Target NameRac1

ConjugateUnconjugated

Other NamesRas-like protein TC25; Ras-related C3 botulinum toxin substrate 1; p21-Rac1

Accession NoSwiss-Prot: P63000
NCBI Protein: NP_008839.2

Uniprot P63000

Gene ID 5879;

Concentration1.0mg/ml

FormulationPBS with 0.05% proclin300,50% glycerol,pH7.3.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 21kd
Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Immunohistochemistry: 1:50-1:200

Immunofluorescence staining of methanol-fixed Hela cells using Rac1(Ab-71) Antibody #21201.
Western blot analysis of extracts from various cells using Rac1(Ab-71) Antibody #21201 .
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue. The picture on the right is blocked with the synthesized peptide.

Rac1 encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Aoki K, et al. (2005) Mol Biol Cell; 16(5): 2207

If you have published an article using product 21201, please notify us so that we can cite your literature.

et al,Type II cyclic guanosine monophosphate-dependent protein kinase inhibits Rac1 activation in gastric cancer cells.In Oncol Lett on 2015 Jul by Ying Wang , Yongchang Chen et al..PMID: 26171059 , (2015),
PMID: 26171059

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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