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Raf1(Ab-338) Antibody#21202

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Product Detail

Product NameRaf1(Ab-338) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Raf1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.336~340 (R-D-S-S-Y) derived from Human RAF.

Target NameRaf1

ConjugateUnconjugated

Other NamesC-RAF; C-Raf; CRAF

Accession NoSwiss-Prot: P04049
NCBI Protein: NP _002871.1

Uniprot P04049

Gene ID 5894;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 73kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells using Raf1(Ab-338) Antibody #21202 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Ab-338) Antibody #21202(left) or the same antibody preincubated with blocking peptide(right).
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

Adams DG,et al. (2005)J Biol Chem. 280(52):42644-54.
Miura S, et al. (2003) Biochem Biophys Res Commun. 306(4):924-9.
Noser JA,et al.(2007)Mol Ther.15(8):1531-6.

If you have published an article using product 21202, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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