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MEK1/MEK2(Ab-217/221) Antibody#21203

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Product Detail

Product NameMEK1/MEK2(Ab-217/221) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total MEK1/MEK2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.215~219/219~223 (I-D-S-M-A) derived from Human MEK1/MEK2.

Target NameMEK1/MEK2

ConjugateUnconjugated

Other NamesERK activator kinase 1/ERK activator kinase2; MAP kinase kinase 1/MAP kinase kinase 2; MAP2K1/MAP2K1; MAPK/ERK kinase 1/MAPK/ERK kinase 2; MAPKK 1/MAPKK 1

Accession NoSwiss-Prot: Q02750
NCBI Protein: NP_002746.1 NP_109587.1

Uniprot Q02750

Gene ID 5604;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 45kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells using MEK1/MEK2(Ab-217/221) Antibody #21203 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MEK1/MEK2(Ab-217/221) Antibody #21203(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using MEK1/MEK2(Ab-217/221) Antibody #21203.
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Shen R, et al. (2002) Mol Cell Biol; 22(10): 3230-3236
Preisinger C, et al. (2005) EMBO J; 24(4): 753-765
Laine P, et al. (2000) Biochem J; 349(Pt 1): 19-25
Yaglom J, et al. (2003) Mol Cell Biol; 23(11): 3813-3824
Dahan S, et al. (2002) Infect Immun; 70(5): 2304-2310

If you have published an article using product 21203, please notify us so that we can cite your literature.

et al,UBIAD1 suppresses the proliferation of bladder carcinoma cells by regulating H-Ras intracellular trafficking via interaction with the C-terminal domain of H-Ras.In Cell Death Dis. On 2018 Dec 5 by Xu Z, Duan F et al..PMID: 30518913 , (2018),
PMID: 30518913
et al,Effect of Chaihushugan San on Expression of the Raf/mitogen-activated Protein Kinase/Extracellular Signal-Regulated Kinase Pathway in the Hippocampi of Perimenopausal Rats Induced by Immobilization Stress .In J Tradit Chin Med on 2015 Aug by Li Shengqiang, Liang Wenna et al..PMID:26427116 , (2015),
PMID: 26427116
et al,Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway. In BMC Microbiol on 2016 Sep 23 by Yanan Li, Zhongjia Jiang,et al..PMID:27663303 , (2016),
PMID: 27663303

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;