Product Detail
Product NameAPP(Ab-668) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total APP protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.666~670 (A-V-T-P-E ) derived from Human APP.
Target NameAPP
ConjugateUnconjugated
Other NamesAAA; AD1; PN2; ABPP; APPI
Accession NoSwiss-Prot: P05067
NCBI Protein: NP_000475.1
Uniprot
P05067
Gene ID
351;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 100-140 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from mouse brain tissue using APP(Ab-668) Antibody #21204 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed MCF cells using APP(Ab-668) Antibody #21204.
APP encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534-542.
Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122
Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085-1091
If you have published an article using product 21204, please notify us so that we can cite your literature.
Yes
