Product Detail
Product NameHSF1(Ab-307) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total HSF1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 305~309 (P-Q-S-P-R) derived from Human HSF1.
Target NameHSF1
ConjugateUnconjugated
Other NamesHSTF 1
Accession NoSwiss-Prot: Q00613
NCBI Protein: NP_005517.1
Uniprot
Q00613
Gene ID
3297;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 82kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela, HepG2 and A2780 cells using HSF1(Ab-307) Antibody #21206.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1(Ab-307) Antibody #21206(left) or the same antibody preincubated with blocking peptide(right).
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4
Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026
Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.
If you have published an article using product 21206, please notify us so that we can cite your literature.
et al,Solanesol protects human hepatic L02 cells from ethanol-induced oxidative injury via upregulation of HO-1 and Hsp70.In Toxicol In Vitro on 2015 Apr by Xiangyang Yao, Qin Bai et al..PMID:25645596
, (2015),
PMID:
25645596
Yes
