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FAK(Ab-397) Antibody#21208

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Product Detail

Product NameFAK(Ab-397) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total FAK protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 395~399 (D-D-Y-A-E) derived from Human FAK.

Target NameFAK

ConjugateUnconjugated

Other NamesFADK; PTK2; FAK1; FRNK; PTK2

Accession NoSwiss-Prot: Q05397
NCBI Protein: NP_005598.3

Uniprot Q05397

Gene ID 5747;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 125kd
Western blotting: 1:500~1:1000

Western blot analysis of extract from HepG2, 293, C6, HUVEC and HT29 cells using FAK(Ab-397) Antibody #21208

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

von Sengbusch A, et al. (2005) Am J Pathol ; 166(2): 585-596
Fornaro M, et al. (2000) Mol Biol Cell ; 11(7): 2235-2249
Zhang X, et al. (1999) Proc Natl Acad Sci U S A; 96(16): 9021-9026.

If you have published an article using product 21208, please notify us so that we can cite your literature.

et al,Long non-codIng RNA PVT1 promotes tumor progression by regulatIng the miR-143/HK2 axis In gallbladder cancer.In Mol Cancer on 2019 Mar 2 by Chen J, Yu Y, et al.. PMID: 30825877 , (2019),
PMID: 30931957
et al,Luteolin reduces the invasive potential of malignant melanoma cells by targeting 尾3 integrin and the epithelial-mesenchymal transition.In Acta Pharmacol Sin on 2012 Oct by Ruan JS, Liu YP,et al..PMID:22983392 , (2012),
PMID: 22983392
et al,Inhibition of hypoxia-induced epithelial mesenchymal transition by luteolin in non-small cell lung cancer cells. In Mol Med Rep on 2012 Jul by Junshan Ruan, Lei Zhang, et al..PMID: 22552526 , (2012),
PMID: 22552526
et al,Anticancer Effect of Heparin-Taurocholate Conjugate on Orthotopically Induced Exocrine and Endocrine Pancreatic Cancer , (2021),
PMID: 34830928

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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