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b-Catenin(Ab-37) Antibody#21212

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Product Detail

Product Nameb-Catenin(Ab-37) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total b-Catenin protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 35~39 (I-H-S-G-A) derived from Human b-Catenin.

Target Nameb-Catenin

ConjugateUnconjugated

Other NamesCTNNB1; CATNB; CTNB1; CTNNB;

Accession NoSwiss-Prot: P35222
NCBI Protein: NP_001091679.1

Uniprot P35222

Gene ID 1499;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 92kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HT29 and SKOV3 cells using b-Catenin(Ab-37) Antibody #21212.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Ab-37) Antibody #21212(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using b-Catenin(Ab-37) Antibody #21212.
Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

If you have published an article using product 21212, please notify us so that we can cite your literature.

et al,Expression of β-catenin in regenerating renal tubules of cisplatin-induced kidney failure in rats .In Clin Exp Nephrol.On 2018 Dec by Terada N, Karim MR et al..PMID:29802483 , (2018),
PMID: 29802483
et al,A Study of the Relationship Between the Wnt/尾-catenin Signaling Pathway and the Gastrointestinal Development of Rat Embryonic and Perinatal Periods.In Exp Ther Med on 2013 Jun by Botao Wei, Yongsheng Guo, et al..PMID:23837038 , (2013),
PMID: 23837038
et al,PC锟?07, a celecoxib derivative, inhibited the growth of colorectal tumor in vitro and in vivo. In Cancer Sci on 2009 Dec by Yuhua Li, Yinbo Niu, et al..PMID: 19814734 , (2009),
PMID: 19814734
et al,Involvement of SET in the Wnt signaling pathway and the development of human colorectal cancer.In Oncol Lett.On 2014 Apr by LEI DONG, JIANJUN ZHU et al..PMID: 24944693 , (2014),
PMID: 24944693
et al,SDF-1/CXCR4 promotes F5M2 osteosarcoma cell migration by activating the Wnt/尾-catenin signaling pathway.In Med Oncol on 2015 Jul by Yao Lu, Bin Hu et al..PMID:26026718 , (2015),
PMID: 26026718
et al,A lentivirus-mediated miR-23b sponge diminishes the malignant phenotype of glioma cells in vitro and in vivo.In Oncol Rep on 2014 Apr Luyue Chen , Kailiang Zhang et al..PMID:24503899 , (2014),
PMID: 24503899
et al,Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis. In Front Cell Dev Biol on 2020 Nov 25 by Guangying Cui, Huifen Wang, et al..PMID:33324633 , (2020),
PMID: 33324633

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;