Product Detail
Product NameEGFR(Ab-1172) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total EGFR protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 1170~1174 (P-D-Y-Q-Q) derived from Human EGFR.
Target NameEGFR
ConjugateUnconjugated
Other NamesReceptor tyrosine-protein kinase ErbB-1
Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2
Uniprot
P00533
Gene ID
1956;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HUVEC cells using EGFR(Ab-1172) Antibody #21213.
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Noguchi T, et al. (1994) Mol Cell Biol; 14(10): 6674-6682
Doherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96(19): 10869-10874
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
O
If you have published an article using product 21213, please notify us so that we can cite your literature.
et al,ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect.In Nat Cell Biol on 2012 Dec by
Weiwei Yang, Yanhua Zheng,et al..PMID:23178880
, (2012),
PMID:
23178880
et al,ZD6474, a new treatment strategy for human osteosarcoma, and its potential synergistic effect with celecoxib銆侷n Oncotarget on 2015 Aug 28 by Jiani Liu, Jiangxue Wu et al..PMID: 26050198
, (2015),
PMID:
26050198
Yes
