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4E-BP1(Ab-46) Antibody#21216

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Product Detail

Product Name4E-BP1(Ab-46) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total 4E-BP1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.44~48 (S-T-T-P-G) derived from Human 4E-BP1.

Target Name4E-BP1

ConjugateUnconjugated

Other NamesEIF4EBP1; PHAS-1;

Accession NoSwiss-Prot: Q13541
NCBI Protein: NP_004086.1

Uniprot Q13541

Gene ID 1978;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 and HepG2 cells using 4E-BP1 (Ab-46) Antibody #21216.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-46) Antibody #21216 (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed Hela cells using 4E-BP1 (Ab-46) Antibody #21216.

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

If you have published an article using product 21216, please notify us so that we can cite your literature.

et al,The AMPK-dependent inhibition of autophagy plays a crucial role in protecting photoreceptor from photooxidative injury In J Photochem Photobiol B On2023 AugbyYu-Lin Li , Tian-Zi Zhang et al..PMID:37302163 , (2023),
PMID: 37302162

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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