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PDGF Receptor b(Ab-751) Antibody#21219

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Product Detail

Product NamePDGF Receptor b(Ab-751) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Rt

SpecificityThe antibody detects endogenous level of total PDGF Receptor b protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.749~753 (V-D-Y-V-P) derived from Human PDGFRb.

Target NamePDGF Receptor b

ConjugateUnconjugated

Accession NoSwiss-Prot: P09619
NCBI Protein: NP_002600.1

Uniprot P09619

Gene ID 5159;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 190kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 cells using PDGF Receptor b(Ab-751) Antibody #21219.

PDGF Receptor b encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor a and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

Lederle W, et al. (2006) Am J Pathol ; 169(5): 1767-1783.
Vignais ML, et al. (1999) Mol Cell Biol; 19(5): 3727-3735.
Herrlich A, et al. (1998) Proc Natl Acad Sci U S A; 95(15): 8985-8990.
Heuchel R, et al. (1999) Proc Natl Acad Sci U S A; 96(20): 11410-11415.

If you have published an article using product 21219, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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