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Met(Ab-1234) Antibody#21220

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Product Detail

Product NameMet(Ab-1234) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Met protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.1232~1236 (K-E-Y-Y-S) derived from Human Met.

Target NameMet

ConjugateUnconjugated

Other NamesHGF receptor; HGF-SF receptor; Met proto-oncogene tyrosine kinase; c-met; kinase Met

Accession NoSwiss-Prot: P08581
NCBI Protein: NP_000236.2

Uniprot P08581

Gene ID 4233;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 156kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HepG2 cells using Met(Ab-1234) Antibody #21220 and the same antibody preincubated with blocking peptide.
Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.

Gherardi E. et al. (2003).Proc Natl Acad Sci U S A. 100(21): 12039-12044.
Shiu SH. et al. (2001) Proc Natl Acad Sci U S A. 98(19): 10763-10768.
Hughes AL. et al. (2001) Genome Res. 11(5): 771-780.
Onuchic LF. et al. (2002) Am J Hum Genet. 70(5): 1305-1317.

If you have published an article using product 21220, please notify us so that we can cite your literature.

et al,MicroRNA-449a Is Downregulated in Non-Small Cell Lung Cancer and Inhibits Migration and Invasion by Targeting c-Met. , (2013),
PMID: 23734217
et al,miR-34a inhibits migration and invasion by down-regulation of c-Met expression in human hepatocellular carcinoma cells. , (2008),
PMID: 19006648
et al,miR-34a Inhibits Migration and Invasion by Down-Regulation of c-Met Expression in Human Hepatocellular Carcinoma Cells.In Cancer Lett on 2009 Mar 8 by Na Li, Hanjiang Fu,et al..PMID:19006648 , (2009),
PMID: 19006648
et al,MicroRNA-449a is downregulated in non-small cell lung cancer and inhibits migration and invasion by targeting c-Met. In PLoS One on 2013 May 29 by Wenting Luo, Bo Huang, et al..PMID: 23734217 , (2013),
PMID: 23734217

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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