Product Detail
Product NameEGFR(Ab-1197) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total EGFR protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.1195~1199 (A-E-Y-L-R) derived from Human EGFR.
Target NameEGFR
ConjugateUnconjugated
Other Names Receptor tyrosine-protein kinase ErbB-1; ERBB1;
Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2
Uniprot
P00533
Gene ID
1956;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HUVEC and MDA cells using EGFR(Ab-1197) Antibody #21221.
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia
Corbalan-Garcia S, et al. (1996) Mol Cell Biolt; 16(10): 5674-5682
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
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If you have published an article using product 21221, please notify us so that we can cite your literature.
et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861
, (2019),
PMID:
31594861
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