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Location： Home > Polyclonal Antibodies > EGFR(Ab-869) Antibody

EGFR(Ab-869) Antibody#21222

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Product Detail

Product NameEGFR(Ab-869) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total EGFR protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.867~871 (K-E-Y-H-A) derived from Human EGFR.

Target NameEGFR

ConjugateUnconjugated

Other Names Receptor tyrosine-protein kinase ErbB-1; ERBB1;

Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2

Uniprot P00533

Gene ID 1956;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Ab-869) Antibody #21222(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of lysates from HUVEC, HUVEC EGF and Hela EGF,using EGFR Antibody.

Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia

Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

If you have published an article using product 21222, please notify us so that we can cite your literature.

et al,Role of Proprotein Convertases in Prostate Cancer Progression.In Neoplasia on 2012 Nov by Fr茅d茅ric Couture, Fran莽ois D'Anjou,In Neoplasia on 2012 Nov by Fr茅d茅ric Couture, Fran莽ois D'Anjou, et al..PMID:23226097 , (2012),
PMID: 23226097
et al,EGFR-induced and PKC蔚 monoubiquitylation-dependent NF-魏B activation upregulates PKM2 expression and promotes tumorigenesis. In Mol Cell on 2012 Dec 14 by Weiwei Yang, Yan Xia, et al..PMID: 23123196 , (2012),
PMID: 23123196
et al,sZIP, an alternative splice variant of ZIP, antagonizes transcription repression and growth inhibition by ZIP. In J Biol Chem on 2010 May 7 by Wenhua Yu, Ruifang Li, et al..PMID: 20233718 , (2010),
PMID: 20233718
et al,Aldosterone induces myofibroblast EGF secretion to regulate epithelial colonic permeability. In Am J Physiol Cell Physiol on 2013 May 1 by Llu茂sa Mir贸, Anna P茅rez-Bosque, et al..PMID:23467299 , (2013),
PMID: 23467299
et al,Secreted and O-GlcNAcylated MIF binds to the human EGF receptor and inhibits its activation.In Nat Cell Biol on 2015 Oct by Yanhua Zheng, Xinjian Li et al..PMID:26280537 , (2015),
PMID: 26280537

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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