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S6 Ribosomal Protein(Ab-235) Antibody#21225

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Product Detail

Product NameS6 Ribosomal Protein(Ab-235) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total S6 Ribosomal protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein.

Target NameS6 Ribosomal Protein

ConjugateUnconjugated

Other NamesNP33; RPS6; RS6

Accession NoSwiss-Prot: P62753
NCBI Protein: NP_001001.2

Uniprot P62753

Gene ID 6194;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 32kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HepG2 and 293 cells using S6 Ribosomal Protein(Ab-235) Antibody #21225.
Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using S6 Ribosomal Protein(Ab-235) Antibody #21225(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using S6 Ribosomal Protein(Ab-235) Antibody #21225.
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

McBride K, et al. (1998) Mol Cell Biol 18(9): 5073-5081.
Williams AJ, et al. (2003) Plant Physiol 132(4): 2086-2097.
Wilson MA, et al. (1997) Biochem J 325(Pt 1): 217-222.
Arnesen T, et al. (2005) Biochem J 386(Pt 3): 433-443.

If you have published an article using product 21225, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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