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eIF4E(Ab-209) Antibody#21226

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Product Detail

Product NameeIF4E(Ab-209) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total elF4E protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E.

Target NameeIF4E

ConjugateUnconjugated

Other NamesmRNA cap-binding protein; eIF-4F 25 kDa subunit;

Accession NoSwiss-Prot: P06730
NCBI Protein: NP_001124150.1

Uniprot P06730

Gene ID 1977;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 25kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela, HepG2 and 293 cells using eIF4E(Ab-209) Antibody #21226.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E(Ab-209) Antibody #21226(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using eIF4E(Ab-209) Antibody #21226.
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Li BD, et al. (1998) Ann Surg; 227(5): 756-763
Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520
De Gregorio E, et al. (2001) RNA; 7(1): 106-113
Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461
Ohlmann T, et al. (1996) EMBO J; 15(6): 1371-1382

If you have published an article using product 21226, please notify us so that we can cite your literature.

et al,Conversion of Leucine to 尾-Hydroxy-尾-Methylbutyrate by 伪-Keto Isocaproate Dioxygenase Is Required for a Potent Stimulation of Protein Synthesis in L6 Rat Myotubes.In J Cachexia Sarcopenia Muscle on 2016 Mar by Mar铆a D Gir贸n , Jos茅 D V铆lchez et al..PMID:27065075 , (2016),
PMID: 27065075
et al,Activation of ERK by sodium tungstate induces protein synthesis and prevents protein degradation in rat L6 myotubes.In FEBS Lett on 2014 Jun 27 by Rafael Salto, Jos茅 D V铆lchez et al..PMID:24846141 , (2014),
PMID: 24846141
et al,Pyruvate Kinase M (PKM) binds ribosomes in a poly-ADP ribosylation dependent manner to induce translational stalling In Nucleic Acids Res On2023 Jul 7byNevraj S Kejiou , Lena Ilan et al..PMID:37224531 , (2023),
PMID: 37224531

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;