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Met(Ab-1349) Antibody#21230

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Product Detail

Product NameMet(Ab-1349) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Met protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.1347~1351 (E-H-Y-V-H) derived from Human Met.

Target NameMet

ConjugateUnconjugated

Other NamesHGF receptor; HGF-SF receptor; Met proto-oncogene tyrosine kinase; c-met; kinase Met

Accession NoSwiss-Prot: P08581
NCBI Protein: NP_000236.2

Uniprot P08581

Gene ID 4233;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 156kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 cells using Met(Ab-1349) Antibody #21230 and the same antibody preincubated with blocking peptide.

Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.

Fan S, et al. (2001) Mol Cell Biol; 21(15): 4968-4984
Schiering N, et al. (2003) Proc Natl Acad Sci U S A; 100(22): 12654-12659
Plopper GE, et al. (1995) Mol Biol Cell; 6(10): 1349-1365
Ponzetto C, et al. (1993) Mol Cell Biol; 13(8): 4600-4608
Jackson PA, et al. (2001) Plant Physiol; 127(3): 1065-1076

If you have published an article using product 21230, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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