Product Detail
Product Namec-Kit(Ab-721) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total c-Kit protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.719~723 (N-E-Y-M-D) derived from Human c-Kit.
Target Namec-Kit
ConjugateUnconjugated
Other NamesKIT; CD117; SCFR; PBT;
Accession NoSwiss-Prot: P11362
NCBI Protein: NP_000213.1
Uniprot
P11362
Gene ID
2260;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 145kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from A431 cells using c-Kit(Ab-721) Antibody #21232 and the same antibody preincubated with blocking peptide.
This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)
Kimura W,et al.(2007)Hepatogastroenterology.54(80):2203-8.
Naeem M,et al.(2002)Hum Pathol. 33(12):1182-7.
Jankulovski N, et al.(2006)Prilozi. 27(2):59-70.
If you have published an article using product 21232, please notify us so that we can cite your literature.
et al,Characterization of Long-Term Cultured c-kit+ Cardiac Stem Cells Derived From Adult Rat Hearts.
, (2010),
PMID:
19580375
Yes
