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eNOS(Ab-495) Antibody#21235

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Product Detail

Product NameeNOS(Ab-495) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsIF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total eNOS protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.493~497 (K-K-T-F-K) derived from Human eNOS.

Target NameeNOS

ConjugateUnconjugated

Other NamesConstitutive NOS; EC-NOS; ECNOS; NOS3; NOSIII

Accession NoSwiss-Prot: P29474
NCBI Protein: NP_000594.2

Uniprot P29474

Gene ID 4846;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 140kd
Immunofluorescence: 1:100~1:200
Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Ab-495) Antibody #21235.
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Moreno H Jr.(2008)Breast Cancer Res Treat. 109(1):181-2.
Lorenz M,et al.(2007)FASEB J. May;21(7):1556-64.
Lin HI,et al.(2006)Transplant Proc. Sep;38(7):2203-6.

If you have published an article using product 21235, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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