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CDC2(Ab-15) Antibody#21236

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Product Detail

Product NameCDC2(Ab-15) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsIF,WB,IHC,ELISA

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total CDC2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 13~17 (G-T-Y-G-V) derived from Human CDC2.

Target NameCDC2

ConjugateUnconjugated

Other NamesCDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;

Accession NoSwiss-Prot: P06493
NCBI Protein: NP_001163877.1

Uniprot P06493

Gene ID 983;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 34kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from Hela and MCF cells using CDC2(Ab-15) Antibody #21236.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2(Ab-15) Antibody #21236(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using CDC2(Ab-15) Antibody #21236.

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Y Gu, et al. (1992) EMBO J. 11(11): 3995

If you have published an article using product 21236, please notify us so that we can cite your literature.

et al,SUMOylation-regulated protein phosphorylation, evidence from quantitative phosphoproteomics analyses.In J Biol Chem on 2011 Aug 5 by Qi Yao, Hui Li,et al..PMID:21685386 , (2011),
PMID: 21685386
et al,Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents. In Biochem J on 2012 Sep 15 by Philip M Kubara, Sophie Kern茅is-Golsteyn, et al..PMID: 22686412 , (2012),
PMID: 22686412
et al,Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I.In Antioxid Redox Signal on 2017 Jul 10 by Xiao-Jing Shi , Lina Ding,et al..PMID: 27650197 , (2017),
PMID: 27650197
et al,Cytotoxic amounts of cisplatin induce either checkpoint adaptation or apoptosis in a concentration鈥恉ependent manner in cancer cells. In Biol Cell on 2016 May by Lucy H Swift, Roy M Golsteyn锛宔t al..PMID:26871414 , (2016),
PMID: 26871414
et al,Isolation of a natural product with anti-mitotic activity from a toxic Canadian prairie plant , (2021),
PMID: 34095597

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;