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p44/42 MAP Kinase(Ab-202) Antibody#21237

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Product Detail

Product Namep44/42 MAP Kinase(Ab-202) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total p44/42 MAP Kinase protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 200~204 (F-L-T-E-Y) derived from Human p44/42 MAP Kinase.

Target Namep44/42 MAP Kinase

ConjugateUnconjugated

Other Names Extracellular signal-regulated kinase 1

Accession NoSwiss-Prot: P27361
NCBI Protein: NP_001035145.1

Uniprot P27361

Gene ID 5595;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 42 44 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 
Western blot analysis of extracts from Hela and 293 cells using p44/42 MAP Kinase(Ab-202) Antibody #21237.
Immunohistochemical analysis of paraffin-embedded human Colorectal tissue using p44/42 MAP Kinase (Ab-202) Antibody #21237.

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1

TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397
Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162
Jingui Yu, et al. (2005) Anesth Analg 101: 315-321
Hironobu Ihn et al.(2000) Immunology 165: 2149-2155
 

If you have published an article using product 21237, please notify us so that we can cite your literature.

et al,The nuclear import of oncoprotein hepatitis B X-interacting protein depends on interacting with c-Fos and phosphorylation of both proteins in breast cancer cells. In J Biol Chem on 2013 Jun 28 by Yingyi Zhang, Yu Zhao, et al..PMID: 23667255 , (2013),
PMID: 23667255

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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