Product Detail
Product NameSAPK/JNK(Ab-183) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total SAPK/JNK protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.181~185 (M-M-T-P-Y) derived from Human SAPK/JNK.
Target NameSAPK/JNK
ConjugateUnconjugated
Other NamesJNK2
Accession NoSwiss-Prot:P45984
Gene ID:5601
Uniprot
P45984
Gene ID
5601;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 46 54 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela and MCF cells using SAPK/JNK(Ab-183) Antibody #21241.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK(Ab-183) Antibody #21241(left) or the same antibody preincubated with blocking peptide(right).
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782
If you have published an article using product 21241, please notify us so that we can cite your literature.
et al,Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts.
, (2011),
PMID:
20709173
et al,Astragaloside IV Inhibits Isoprenaline鈥慽nduced Cardiac Fibrosis by Targeting the Reactive Oxygen Species/Mitogen鈥慳ctivated Protein Kinase Signaling Axis.In Mol Med Rep on 2017 Apr by Hongliang Dai ,Guizhi Jia ,et al..PMID:28260010
, (2017),
PMID:
28260010
Yes
