Product Detail
Product NameP38 MAPK(Ab-182) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total P38MAPK protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 180~184 (T-G-Y-V-A) derived from Human P38 MAPK.
Target NameP38 MAPK
ConjugateUnconjugated
Other NamesMAPK2; MAPKAPK-2; MAPKAPK2
Accession NoSwiss-Prot: Q16539
NCBI Protein: NP _001306.1
Uniprot
Q16539
Gene ID
1432;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 293 and Hela cells using P38 MAPK(Ab-182) Antibody #21245.
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Ming Zheng, et al.(2005) The FASEB Journal. 19: 109-111
Bernt van den et al.(2001) Blink Immunology, 166: 582-587
Arshad Rahman, et al. (2004) Am J Physiol Lung Cell Mol Physiol 287: L1017-L1024
Osamu Yoshino, et al. (2003) Endocrinology & Metabolism Vol. 88: 2236-2241
If you have published an article using product 21245, please notify us so that we can cite your literature.
et al,Attenuating phosphorylation of p38 MAPK in the activated microglia: A new mechanism for intrathecal lidocaine reversing tactile allodynia following chronic constriction injury in rats.
, (2008),
PMID:
18191894
et al,In vitro-induced M2 type macrophages induces the resistance of prostate cancer cells to cytotoxic action of NK cells.In Exp Cell Res.On 2018 Mar 1 by Xu L, Shen M et al..PMID: 29408565
, (2018),
PMID:
29408565
et al,Neuroendocrine differentiation contributes to radioresistance development and metastatic potential increase in non-small cell lung cancer.In Biochim Biophys Acta Mol Cell Res. On 2018 Dec by Zhu R, Yang X et al..PMID: 30262435
, (2018),
PMID:
30262435
et al,Radiation Alters PD-L1/NKG2D Ligand Levels in Lung Cancer Cells and Leads to Immune Escape From NK Cell Cytotoxicity via IL-6-MEK/Erk Signaling Pathway.In Oncotarget on 2017 Jul 12 by Ming Jing Shen, Li Jun Xu,et al..PMID:29113321
, (2017),
PMID:
29113321
et al,A solute carrier family 22 member 3 variant rs3088442 G锟?A associated with coronary heart disease inhibits lipopolysaccharide-induced inflammatory response. In J Biol Chem on 2015 Feb 27 by Lu Li, Meian He,et al..PMID:25561729
, (2015),
PMID:
25561729
Yes
