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Connexin 43(Ab-368) Antibody#21250

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Product Detail

Product NameConnexin 43(Ab-368) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Connexin43 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 366~370 (R-A-S-S-R) derived from Human Connexin 43.

Target NameConnexin 43

ConjugateUnconjugated

Other NamesCX43; CXA1; CXN-43; Connexin 43; GJA1

Accession NoSwiss-Prot: P17302
NCBI Protein: NP_000156.1

Uniprot P17302

Gene ID 2697;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HT29 and HepG2 cells using Connexin 43 (Ab-368) Antibody #21250.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43 (Ab-368) Antibody #21250 (left) or the same antibody preincubated with blocking peptide (right).

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Joell L. Solan1, et al. (2003) Cell Science 116: 2203-2211
Satoshi Matsushita, et al. (2006) Histochemistry and Cytochemistry 54 (3): 343-353,
Xiaoyong Bao, et al. (2004) Cell Physiol 286: C647-C654
W. E. I. Li, et al.(1998) European Journal of Neuroscience 10: 2444

If you have published an article using product 21250, please notify us so that we can cite your literature.

et al,The engineered thymidylate kinase (TMPK)/AZT enzyme-prodrug axis offers efficient bystander cell killing for suicide gene therapy of cancer. In PLoS One on 2013 Oct 23 by Takeya Sato, Anton Neschadim, et al..PMID:24194950 , (2013),
PMID: 24194950

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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