Product Detail
Product NameEGFR(Ab-1110) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total EGFR protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 1108~1112 (P-V-Y-H-N) derived from Human EGFR.
Target NameEGFR
Other Names Receptor tyrosine-protein kinase ErbB-1
Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2
Uniprot
P00533
Gene ID
1956;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HUVEC and A431 cells using EGFR(Ab-1110) Antibody #21256.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Ab-1110) Antibody #21256(left) or the same antibody preincubated with blocking peptide(right).
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Kaisa Erjala, et al. (2006) Clin. Cancer Res Jul 2006; 12: 4103 - 4111.
Judit Anido, et al. (2003) Clin. Cancer Res ; 9: 1274.
Julian Andreev, et al. (2001) J. Biol. Chem ; 276: 20130 - 20135.
Suil Kim, et al. (2002) Am J Physiol Lung Cell Mol Physiol; 283: 67.
If you have published an article using product 21256, please notify us so that we can cite your literature.
Yes
