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MARCKS(Ab-162) Antibody#21257

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Product Detail

Product NameMARCKS(Ab-162) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total MARCKS protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 160~164 (K-K-S-F-K) derived from Human MARCKS.

Target NameMARCKS

ConjugateUnconjugated

Other NamesMACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate

Accession NoSwiss-Prot: P29966
NCBI Protein: NP_002347.5

Uniprot P29966

Gene ID 4082;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela and HT29 cells using MARCKS(Ab-162) Antibody #21257.
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159

If you have published an article using product 21257, please notify us so that we can cite your literature.

et al,The GABAB positive allosteric modulators CGP7930 and GS39783 stimulate ERK1/2 signalling in cells lacking functional GABAB receptors.In Eur J Pharmacol on 2017 Jan 5 by Maria C Olianas, Simona Dedoni,et al..PMID: 27876620 , (2017),
PMID: 27876620
et al,Protection from interferon鈥愇测€恑nduced neuronal apoptosis through stimulation of muscarinic acetylcholine receptors coupled to ERK1/2 activation.In Br J Pharmacol on 2016 Oct by Maria C Olianas, Simona Dedoni et al..PMID:27474091 , (2016),
PMID: 27474091

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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