Product Detail
Product NameHistone H2A.X(Ab-139) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total Histone H2A.X protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.
Target NameHistone H2A.X
ConjugateUnconjugated
Other NamesH2A.X; H2AFX; H2a/x; HIST5-2AX;
Accession NoSwiss-Prot: P16104
NCBI Protein: NP_002096.1
Uniprot
P16104
Gene ID
3014;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 15kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation
Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6
If you have published an article using product 21260, please notify us so that we can cite your literature.
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et al,Anti-inflammatory mechanism of taurine against ischemic stroke is related to down-regulation of PARP and NF-魏B. In Amino Acids on 2012 May by Ming Sun, Yumei Zhao, et al..PMID: 21409386
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et al,BASI, A Potent Small Molecular Inhibitor, Inhibits Glioblastoma Progression by Targeting microRNA鈥恗ediated 尾鈥怌atenin Signaling.In CNS Neurosci Ther on 2014 Sep by Zhen-Dong Shi , Xiao-Min Qian et al..PMID: 24810017
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Yes
