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p70 S6 Kinase(Ab-411) Antibody#21261

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Product Detail

Product Namep70 S6 Kinase(Ab-411) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total p70 S6 Kinase protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 409~413 (I-R-S-P-R) derived from Human p70 S6 Kinase.

Target Namep70 S6 Kinase

ConjugateUnconjugated

Other NamesKS6B1; P70-S6K; RPS6KB1; S6K;

Accession NoSwiss-Prot: P23443
NCBI Protein: NP_003152.1

Uniprot P23443

Gene ID 6198;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 70 85 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 cells using p70 S6 Kinase(Ab-411) Antibody #21261.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Ab-411) Antibody #21261(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase(Ab-411) Antibody #21261.
Western blotting analysis using p70 S6 Kinase(Ab-411) Antibody #21261.

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851
Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.
Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c
Le, X.F, et al. (2003) Oncogene 22: 484

If you have published an article using product 21261, please notify us so that we can cite your literature.

et al, miR-223 reverses the resistance of EGFR-TKIs through IGF1R/PI3K/Akt signaling pathway.In Int J Oncol.On 2016 May by J Han, F Zhao et al..PMID: 26936292 , (2016),
PMID: 26936292

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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